Coding

Part:BBa_K4823011:Design

Designed by: Zike Yao   Group: iGEM23_HKSSC   (2023-10-10)


CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

CcdB is able to kill E. coli, therefore we have designed an IPTG inducible promoter before the CcdB coding. It is only after the cells have been cultured and collected, then IPTG will be added into the cell culture, to induce the expression of CcdB gene under the powerful T7 promoter.


Source

Arabidopsis thaliana

References

Gourav S., iGEM20_BITSPilani-Goa_India(2020). BBa_K3512001, Registry of Standard Biological Parts, https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=BBa%20K3512002